Connected on 2014-09-23 09:00:00 from Richmond County, New York, United States
- Bugscope Team microscope is p[umpiong\
- Bugscope Team microscope is pumping down
- Teacher good morning! thank you for getting setup so early!
- Bugscope Team good morning!
- Bugscope Team we still have to make the presets for your session. I'll start now
- Teacher no problem, no kids until 930
- Teacher do you have to coat the specimens in gold before they are put under the SEM?
- Bugscope Team yes. we use gold-palladium, which gives a finer coat.
- Teacher Scott, what is your role? Are you a post doc student? What did you study during your graduate career? I know the kids will be as interested in who you are as what we are looking at!
- Bugscope Team I manage the Microscopy Suite; we train mostly grad students and postdocs to run a variety of instruments in order to conduct their own research. I was hired here in late 1998 to run this microscope and to help start Bugscope.
- Bugscope Team Prior to that I'd run an EM facility in Kansas City for 10+ years, and before that a lab at the U of Kansas for 5 years.
- Teacher Very cool. My masters thesis was using XRay Fluorescence Spectroscopy at SUNY Albany to study prehistoric stone tools
- Teacher Any idea what species of moth you are using?
- Bugscope Team not today; when the entomologists log in this morning they may be able to help us with that
- Bugscope Team lobed antenna
- Bugscope Team we are ready to roll.
- Teacher great scott, the kids are here we are just talking about the SEM
- Bugscope Team you can now drive; please let us know when you have questions
- Bugscope Team If you would like, we can confer control to students, one at a time...
- Bugscope Team this is Scott, again, on my office computer.
- Bugscope Team you have control of the microscope
- Bugscope Team super cool
- Bugscope Team here you can see the mandibles, which open side to side, and the palps
- Bugscope Team also the compound eyes, of course
- Bugscope Team the antennae are broken off
- Bugscope Team there are three more eyes we cannot see on the top of the head -- the ocelli, or simple eyes
- Bugscope Team this is the opening of the stink gland, between the first and second set of legs on the ventral side
- Bugscope Team a lot of fluid had come out of the gland, and the gold-palladium did not stick well to that area
- Bugscope Team we use the gold-palladium to make the surface of the sample conductive
- Bugscope Team and we coat extra thick for Bugscope since it's not for research and the individual features are hard to render conductive
- Bugscope Team please be sure to let us know when you have questions about anything
- Teacher is it 24k gold?
- Bugscope Team I think it's 70% pure gold and 30% pure palladium.
- Bugscope Team it appears silvery
- Bugscope Team if you coat with gold only, the coating is chunky, and with a high-resolution SEM we can see that chunkiness, like a late 1960's sculpture.
- Bugscope Team gold-palladium coats as flakes, kind of -- it looks like dried mud up close
- Bugscope Team here we see where the sheath of the ovipositor has sprung away, and the stinger/ovipositor is exposed.
- Bugscope Team you can change the mag, and also if you click on the screen, the image will center where you click
- Bugscope Team you can see one of the lower limbs
- Teacher how do we change mag?
Bugscope Team You can click on the + or - buttons at the top of the image, next to the word "Magnification". Similarly for focus, and image quality adjustments.
- Bugscope Team we did not look to see if we could find any hamuli, which are the hooks between the hind- and forewing
- Bugscope Team that is odd...
- Bugscope Team we can make changes for you as well, of course
- Teacher can we come out a bit?
- Bugscope Team this is as low as we can get with this setup
- Teacher they are dying to know who daniel is
Bugscope Team Some boring old dude who has been part of this since the beginning in 1999. Took a break to do something stupid (got a Ph.D.) and am now an unemployed bum sponging off my wife. :)
- Teacher not seeing + - next to mag, justn ext to focus and contrast, but thats ok
Bugscope Team It is to the right of the word. The top row should be read like this: "Magnification: +/-. Focus: +/-. Etc." The spacing may make it seem a bit odd. Or are those not there? If not, then let me know and I'll try to figure out what happened.
Bugscope Team The red "+" should be just under the word "the" at the top of the page where it says "Currently near the assassin bug".
- Teacher why are they called "assasin bugs"?
Bugscope Team they blend in well with their surroundings and then when an insect they want to eat comes by, they grab them with their front legs and pierce the insect with their mouthpart and suck out the juices
Bugscope Team They are very good predators, as Cate just said.
- Bugscope Team the features we're looking at now are on the nano scale
- Teacher and what exactly is a ommatidia?
Bugscope Team they are these- the individual facets of the compound eye.
- Bugscope Team Hello Reynolds!
- Bugscope Team Welcome to Bugscope!
- Bugscope Team We're working with the Staten Island Academy this morning.
- Bugscope Team please let us know if you have any questions for us
- Teacher Cate, the kids know I love moths and we have been studying them. Can you tell us the species of moth we have today?
Bugscope Team I'm sorry. I have no idea.
Bugscope Team I wasn't part of the specimen prep, so I didn't get a look at it before it went into the chamber. Based on its size, I'm guessing it is probably a European corn borer moth, Ostrinia nubilalis Huber. We see a lot of them this time of year as the second brood of caterpillars has pupated and become adults.
- Bugscope Team this is the wasp's claw, and in between the 'tines' we see what is sometimes called an 'arolium' in grasshoppers, for example
- Teacher What are the induvidual hair like structures called?
Bugscope Team setae, bristles, spines, trichae, microtrichae, microsetae, and then just hairs again
Bugscope Team The list the Scot provided is just a sampling. The specific term used varies depending upon anatomy and function.
- Teacher Can you look at rocks and non living objects with it?
Bugscope Team all the time!
- Bugscope Team we look at biofilms, microcapsules, all kinds of micro and nanofabricated devices
- Teacher Do you ever use living specimens?
Bugscope Team they are not happy to be in low vacuum, so we don't if we can help it
- Bugscope Team this is a fairly well-equipped microscopy suite, so we have all kinds of other image and spectra collecting devices
- Bugscope Team we have transmission electron microscopy, atomic force microscopy, fluorescence microscopy of many different types, microCT, nanoCT, laser scanning confocal microscopy, Raman imaging....
- Bugscope Team it has some juju on it
- Bugscope Team oh yeah as Daniel says this is an ESEM, which means we can image samples without coating, as well
- Bugscope Team diatom!
Bugscope Team Mmmm... Jelly donut.
- Teacher is this the highest magnification possible?
Bugscope Team For Bugscope, generally yes. For the scope, not even close.
Bugscope Team no we can go much higher, but the way we have the 'scope set up today, we have limited our resolution a bit
Bugscope Team We limit the upper magnification somewhat because it is hard to focus when the magnification gets too high.
- Teacher 10 microns?
Bugscope Team A micron is one millionth of a meter. Just as a millimeter is 1/1000th, a micron (technically a micrometer, but micron is the accepted shorthand) is 1/1,000,000th of a meter. In other words, 1/1000th of a millimeter.
- Bugscope Team when we're running Bugscope we have the sample more than an inch away from the pole piece, which allows us to go as low as 37x or so but not as high as 200,000x and still get good images.
- Teacher Thank you so much for your time today, the kids absolutely loved it. Can the kids log in at home to continue exploring these images?
- Bugscope Team and we can go over 200,000x as well
- Bugscope Team http://bugscope.beckman.illinois.edu/members/2014-071
- Teacher what is a diatom?
Bugscope Team it's a silica-shelled, single cell algae
Bugscope Team or I've heard them described as algae...
Bugscope Team Yes, they are photosynthetic algae. They build translucent silica shelles around their bodies and can be quite beautiful. They also persist for a long time in the soil after dying, so form an important clue in the fossil record about the environmental conditions of the region.
- Bugscope Team Reyn olds
- Bugscope Team Thank you! The link below is to today's session -- it has a transcript and some of the images
Bugscope Team NOTE, that link pasted into the transcript below lacks the ":" between "http" and "//". It should read "http : //" (without the spaces) but a bug in the transcript software strips it out. We haven't found where that is happening yet, so we haven't been able to fix it.
- Bugscope Team Reynolds Science (sorry I can hardly type) please let us know if you have any questions.
- Teacher Thanks again!
Bugscope Team It was fun having you! We hope you want to do this again!
- Bugscope Team SIA if you want to drive any more you may -- all of the images go to your school's member page for today
- Teacher do you burn the specimens to get the gold back?
Bugscope Team The Scot is saving them up to make a gold tooth! (Not really.)
Bugscope Team it is so thin it's not worth messing with
Bugscope Team It's only a few atoms thick, so it would take several truckloads of coated bugs to be worthwhile. :)
- Bugscope Team Please feel free to continue to drive around if you like.
- Teacher thanks, i will
Bugscope Team Did the mag +/- buttons every show up? If not, what browser and browser version are you running? We like to know this information so we can find bugs and fix them for future schools and sessions.
- Bugscope Team we're shutting down.. Thank you, Everyone!
- Bugscope Team Dave... my mind is going... I can feel it...